Simultaneous cellulose degradation and electricity production by Enterobacter cloacae in a microbial fuel cell.
نویسندگان
چکیده
Electricity can be directly generated by bacteria in microbial fuel cells (MFCs) from many different biodegradable substrates. When cellulose is used as the substrate, electricity generation requires a microbial community with both cellulolytic and exoelectrogenic activities. Cellulose degradation with electricity production by a pure culture has not been previously demonstrated without addition of an exogenous mediator. Using a specially designed U-tube MFC, we enriched a consortium of exoelectrogenic bacteria capable of using cellulose as the sole electron donor. After 19 dilution-to-extinction serial transfers of the consortium, 16S rRNA gene-based community analysis using denaturing gradient gel electrophoresis and band sequencing revealed that the dominant bacterium was Enterobacter cloacae. An isolate designated E. cloacae FR from the enrichment was found to be 100% identical to E. cloacae ATCC 13047(T) based on a partial 16S rRNA sequence. In polarization tests using the U-tube MFC and cellulose as a substrate, strain FR produced 4.9 +/- 0.01 mW/m(2), compared to 5.4 +/- 0.3 mW/m(2) for strain ATCC 13047(T). These results demonstrate for the first time that it is possible to generate electricity from cellulose using a single bacterial strain without exogenous mediators.
منابع مشابه
Pollution reduction and electricity production from dairy industry wastewater with microbial fuel cell
Taguchi L9 orthogonal array was implemented to select optimum values of process parameters and to attain the maximum removal of pollutants and power generation from dairy industry wastewater using double chambered salt bridge microbial fuel cell. The maximum chemical oxygen demand reduction, current, voltage, power, current density and power density in double chambered salt bridge microbial fue...
متن کاملHow firebrats (Thysanura: Lepismatidae) detect and nutritionally benefit from their microbial symbionts Enterobacter cloacae and Mycotypha microspora.
The phylogenetically ancient firebrats, Thermobia domestica (Packard) (Thysanura: Lepismatidae), lack any form of long-distance communication, yet are able to locate mates in sustained hot and humid microhabitats, typically within human habitations where they feed on dried goods, including cellulosic substrates. We have recently shown that firebrats aggregate in response to two symbiotic microo...
متن کاملElectricity production from cellulose in a microbial fuel cell using a defined binary culture.
Microbial fuel cells (MFCs) convert biodegradable materials into electricity, potentially contributing to an array of renewable energy production strategies tailored for specific applications. Since there are no known microorganisms that can both metabolize cellulose and transfer electrons to solid extracellular substrates, the conversion of cellulosic biomass to electricity requires a syntroph...
متن کاملMulti-time series RNA-seq analysis of Enterobacter lignolyticus SCF1 during growth in lignin-amended medium
The production of lignocellulosic-derived biofuels is a highly promising source of alternative energy, but it has been constrained by the lack of a microbial platform capable to efficiently degrade this recalcitrant material and cope with by-products that can be toxic to cells. Species that naturally grow in environments where carbon is mainly available as lignin are promising for finding new w...
متن کاملApplication of dual chamber microbial fuel cell with aeration cathode for bioelectricity generation and simultaneous industrial wastewater treatment
Background and Objective: Microbial fuel cell (MFC) is a new green technology that uses the catabolic ability of microorganisms to produce bioenergy while simultaneously removing organic matter and other wastewater contaminants. Electrode material is one of the factors affecting the performance of microbial fuel cells. The aim of this study was to investigate the performance of microbial fuel c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 75 11 شماره
صفحات -
تاریخ انتشار 2009